Chair for Computer Aided Medical Procedures & Augmented Reality
Lehrstuhl für Informatikanwendungen in der Medizin & Augmented Reality

THIS WEBPAGE IS DEPRECATED - please visit our new website

S. Albarqouni, T. Lasser, W. Alkhaldi, A. Al-Amoudi, N. Navab
Gradient Projection for Regularized Cryo-Electron Tomographic Reconstruction
Proceedings of MICCAI Workshop on Computational Methods for Molecular Imaging, Boston, USA, September 2014. (bib)

Cryo-ET has recently emerged as a leading technique to investigate the three-dimensional (3D) structure of biological specimens at close-to-native state. The technique consists of acquiring many two-dimensional (2D) projections of the structure under scrutiny at various tilt angles under cryogenic conditions. The 3D structure is recovered through a number of steps including projection alignment and reconstruction. However, the resolution currently achieved by cryo-ET is well below the instrumental resolution mainly due to the contrast transfer function of the microscope, the limited tilt range and the high noise power. These limitations make the 3D reconstruction procedure very challenging. Here, we propose a new regularized reconstruction technique based on projected gradient algorithm. Using the gold-standard method for resolution assessment, the Fourier Shell Correlation, we show that the proposed technique outperforms the commonly used reconstruction methods in ET, including the filtered back projection and the algebraic reconstruction techniques.
This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each authors copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.

Edit | Attach | Refresh | Diffs | More | Revision r1.13 - 30 Jan 2019 - 15:16 - LeslieCasas

Lehrstuhl für Computer Aided Medical Procedures & Augmented Reality    rss.gif