Chair for Computer Aided Medical Procedures & Augmented Reality
Lehrstuhl für Informatikanwendungen in der Medizin & Augmented Reality

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A. Vahadane, T. Peng, S. Albarqouni, M. Baust, K. Steiger, A. M. Schlitter, A. Sethi, I. Esposito, N. Navab
Structure-Preserved Color Normalization for Histological Images
International Symposium on Biomedical Imaging (ISBI): From Nano to Macro, New York, USA, April 2015 (bib)

Automated image processing and quantification are increasingly gaining attention in the field of digital pathology. However, a common problem that encumbers computerized analysis is the color variation in histology, due to the use of different microscopes/scanners, or inconsistencies in tissue preparation. In this paper, we present a novel color normalization technique to bring a histological image (source image) into a different color appearance of a second image (target image), which therefore standardizes the color representation of both images. In particular, by incorporating biological stain-sparse regularized stain separation, our color normalization technique preserves the structural information of the source image after color normalization, which is very important for subsequent image analysis. Both qualitative and quantitative validation demonstrates the superior performance of our stain separation and color normalization techniques
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