Chair for Computer Aided Medical Procedures & Augmented Reality
Lehrstuhl für Informatikanwendungen in der Medizin & Augmented Reality

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T. Peng, K. Thorn, T. Schroeder, L. Wang, F. Theis, C. Marr, N. Navab
A BaSiC? Tool for Background and Shading Correction of Optical Microscopy Images
Nature Communications 8:14836 (bib)

Quantitative analysis of bioimaging data is often skewed by both shading in space and background variation in time. We introduce BaSiC?, an image correction method based on low rank and sparse decomposition which solves both issues. In comparison to existing shading correction tools, BaSiC? achieves high accuracy with significantly fewer input images, works for diverse imaging conditions and is robust against artefacts. Moreover, it can correct temporal drift in time-lapse microscopy data and thus improve continuous single-cell quantification. BaSiC? requires no manual parameter setting and is available as a Fiji/ImageJ plugin.
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